A kinetic model for curcumin production in Escherichia coli

Optimization of fermentation conditions for the production of curcumin by engineered Escherichia coli.

Curcumin is a plant secondary metabolite with outstanding therapeutic effects. Therefore, there is a great interest in developing new strategies to produce this high-value compound in a cheaper and environmentally friendly way. Curcumin heterologous production in Escherichia coli using artificial biosynthetic pathways was previously demonstrated using synthetic biology approaches. However, the ...

Production of curcuminoids from tyrosine by a metabolically engineered Escherichia coli using caffeic acid as an intermediate.

Curcuminoids are phenylpropanoids with high pharmaceutical potential. Herein, we report an engineered artificial pathway in Escherichia coli to produce natural curcuminoids through caffeic acid. Arabidopsis thaliana 4-coumaroyl-CoA ligase and Curcuma longa diketide-CoA synthase (DCS) and curcumin synthase (CURS1) were used to produce curcuminoids and 70 mg/L of curcumin was obtained from feruli...

Using L-arabinose for Production of Human Growth Hormone in Escherichia coli, Studying the Processing of gIII::hGH Precursor

Precursor-directed biosynthesis of curcumin analogs in Escherichia coli.

Curcuminoids, natural products in the rhizome of turmeric, show various biological activities, including antioxidant and antitumor activities. For this reason, curcuminoids have been focused on as potential pharmaceuticals. Exogenous supplementation with various carboxylate precursors in genetically engineered Escherichia coli cells carrying an artificially assembled pathway for curcuminoid bio...

Evaluating a murine model of endometritis using uterine isolates of Escherichia coli from postpartum buffalo

Ascending infection of the uterus with Gram-negative bacteria is responsible for postpartum endometritis in cattle and buffalo and can adversely affect fertility. Development of a laboratory animal model for bovine endometritis would facilitate the understanding of the pathogenesis as it is difficult to conduct controlled experimentation in the native host. In the present study, 30 virgin Swiss...